Description :
- Analyze the DNA methylation alterations in fish spermatozoa following cryopreservation, and pinpoint the locations of two species containing two distinct packing proteins (protamines in salmonids and histones in cyprinids) that are known to be fragile.
- Determine whether local adjustments to the chromatin compaction features are related to cryopreservation-induced changes in DNA methylation.
- Determine whether the progeny receives epigenetic changes from cryopreserved spermatozoa.
Results :
- RRBS profile of DNA methylation in rainbow trout and carp spermatozoa some time recently and after cryopreservation in ideal and sub-optimal conditions, and outline of the likenesses and inconsistencies between the two species.
- ATAC seq information from new and cryopreserved spermatozoa and comparative examination between new and cryopreserved tests.
- On the species with the most grounded DNA methylation changes, RRBS profile of the embryonic cells earlier to genome actuation in embryos sired by new or cryopreserved spermatozoa.
Location :
- Fish Physiology and Genomics Unit, French National Research Institute for Agriculture, Food and Environment (INRAE), France
Main Supervisor :
- Dr. Catherine Labbé
- E-mail : catherine.labbe@inrae.fr
Participating researchers :
- Dr. Audrey Laurent
- Dr. Tim Rittinghaus (LUH), Dr. Rosa Pereira (INIAV)
Planned secondments: (Host, Supervisor, timing, length, and purpose)
- INIAV, M15, 1 M, training on mammalian embryo cryopreservation and viability assessment, to gain background in mammals.
- JU (USB), Psenicka, M12, 3 M, training on carp sperm collection and cryopreservation. Preparation of carp samples (sperm and embryos DNA). Gaining insight into the specificities of carp sperm and embryo manipulation.
- LUH, Glasmacher, M24, 1 M, training in Raman spectroscopy and its application to cryobiology, to gain skills in biophysics.
How to Apply :
- You Can apply by Send your CV, motivation letter and names and contact details for referees by e-mail to the Main Supervisor.
