Description :
- Validation of improved protocols for the vitrification of teleost gonadal tissue
Location :
- Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Norway
Main Supervisor :
- Prof. Ian Mayer.
E-mail :
Objectives :
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Develop and validate a method for the vitrification of gonadal tissue from teleost species
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Validate recovery success of frozen/thawed fragments in vitro
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Application of laser cryotome to visualize and characterize freeze-thaw damage in gonadal tissue
Expected Results :
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Establish vitrification protocols for the successful cryopreservation of gonadal tissue.
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Confirm viability of germ cells/primary oocytes harvested from cryopreserved ovarian fragments.
Planned secondments: (Host, Supervisor, timing, length, and purpose) :
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JU (USB), Pšenička, M10, 1 M, vitrification of fish ovarian tissue
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INIAV, Pereira, M14, 3 M, vitrification of bovine ovarian tissue
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LLS Rowiak, Stolze, M22, 1 M, application of laser microscopy for the visualization of freeze-thaw damage in ovarian tissue
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SCBI, Comizzoli, M30, 1 M, learning advanced cryotechnology, including novel methods for ovarian tissue cryopreservation.